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KMID : 1007520220310121501
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Food Science and Biotechnology
2022 Volume.31 No. 12 p.1501 ~ p.1511
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Review in isothermal amplification technology in food microbiological detection
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Li Xiang
Zhang Xiaolong
Shi Xiaoli
Shi Hongfei
Wang Zhouping
Peng Chifang
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Abstract
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Food-borne diseases caused by microbial contamination have always been a matter of great concern to human beings. Hence, the research on these problems has never stopped. With the development of microorganism amplification technology, more and more detection methods have come into our vision. However, traditional detection technologies presents more or less drawbacks, such as complicated operation, low accuracy, low sensitivity, long-time detection, and so on. Therefore, more convenient, accurate, and sensitive measurement for the microorganism are needed. Isothermal amplification technology is one of the alternative approach containing the above mentioned advantages. This work mainly summarizes the principles of loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA) which belong to isothermal amplification. Meanwhile, the application of LAMP and RCA in food microorganism detection is introduced.
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KEYWORD
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Food safety, Isothermal amplification, LAMP, RCA, Microorganism, Analytical method
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